Rabbit Anti-APG8a/b(MAP1LC3A/B) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | Q9GZQ8 |
| Gene Id | 81631 |
| Gene Symbols | MAP1LC3B |
| Protein Name / Synonyms | APG8a/b(MAP1LC3A/B) |
| Clonality | Polyclonal |
| Preparation | This Cleaved-APG8a/b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 100-125 amino acids from human Cleaved-APG8a/b. |
| Concentration (lot specific) | 0.290 mg/ml |
| Recommended Applications | IF, Western Blotting, IHC-P |
| Research Area | Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
Specificity
Predicted Reactivity
Bovine
Other Names
MAP1LC3B; MAP1ALC3; Microtubule-associated proteins 1A/1B light chain 3B; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B light chain 3 B; Microtubule-associated protein 1 light chain 3 beta
NCBI Accession #
NP_073729.1
Other Accession #
O41515; NP_073729.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
14688
Recommended Dilutions
IF: 1:100; WB: 1:1000; IHC: 1:50-100
 | Fluorescent image of U251 cells stained with APG8a/b (MAP1LC3A/B) antibody. U251 cells were treated with Chloroquine (50 µM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with 102-10730 APG8a/b (MAP1LC3A/B) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). APG8a/b (MAP1LC3A/B) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells. |
 | APG8a/b (MAP1LC3A/B) (Cat# 102-10730) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the MAP1LC3A antibody detected the MAP1LC3A protein (arrow). |
 | Cleaved-APG8a/b antibody (MAP1LC3A/B) (Cat# 102-10730) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Cleaved-APG8a/b antibody (MAP1LC3A/B) for immunohistochemistry. Clinical relevance has not been evaluated. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.