Synonyms
ARF3 antibody, small GTP binding protein antibody, ADP-ribosylation factor 3 antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
21 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 15 and 181 of ARF3
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000, IHC-P: 1-100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of methanol-fixed HeLa, using ARF3(107-10483) antibody at 1:500 dilution.

ARF3 antibody detects ARF3 protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
ARF3 antibody (107-10483) dilution: 1:500.

ARF3 antibody detects ARF3 protein at cytosol on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
ARF3 antibody (107-10483) dilution: 1:500.

Sample (30 ug of whole cell lysate)
A: Raji
12% SDS PAGE
ADP-ribosylation factor 3 antibody
107-10483 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.