Arginase 1 antibody, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 383 |
| Protein Name / Synonyms | Arginase 1 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
ARG1 antibody, arginase-1 antibody, type I arginase antibody, liver-type arginase antibody, arginase, liver antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
35 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 322 of Arginase I (Uniprot ID# P05089)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:50000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Sample (20 ug of whole cell lysate)
A: mouse liver
10% SDS PAGE
107-10825 diluted at 1:50000
A: mouse liver
10% SDS PAGE
107-10825 diluted at 1:50000
Arginase 1 antibody detects ARG1 protein by Western blot analysis.
A. 30 µg HepG2 whole cell lysate/extract
10 % SDS-PAGE
Arginase 1 antibody (107-10825) dilution: 1:1000
A. 30 µg HepG2 whole cell lysate/extract
10 % SDS-PAGE
Arginase 1 antibody (107-10825) dilution: 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using arginase I (107-10825) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using arginase I (107-10825) antibody at 1:500 dilution.
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.