Mouse Anti-Human ATG12 Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Accession Number | O94817 |
| Gene Id | 9140 |
| Gene Symbols | ATG12 |
| Protein Name / Synonyms | ATG12 |
| Clonality | Monoclonal |
| Preparation | APG12L recombinant protein is used to produce this monoclonal antibody. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting |
| Research Area | Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).
Specificity
Other Names
ATG12; APG12; APG12L; Ubiquitin-like protein ATG12; Autophagy-related protein 12
NCBI Accession #
NP_001264712.1;NP_004698.3
Format
Type
Purified Mouse Monoclonal Antibody (Mab)
Calculated Molecular Weight (Da)
15113
Recommended Dilutions
WB: 1:200-2000; IHC: 1:50-200; IF: 1:10-50
 | Western blot analysis of anti-APG12 Monoclonal Antibody (Cat# 102-10046) by APG12 recombinant protein. APG12(arrow) was detected using the purified Mab. |
 | Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue and lung carcinoma tissue reacted with APG12L Monoclonal Antibody (Cat# 102-10046), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with APG12L Monoclonal Antibody (Cat# 102-10046), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Atg12 antibody tested in HEK cells. Fixed in PFA permeablilized with 0.2% Saponin. Blocked and antibodies in 10% goat serum. Secondary was mouse 555. The right is in full medium (FM) and the left with EBSS (no leupeptin)(Provided by Nicole McKnight & Sharon Tooze). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.