Calsequestrin-2 antibody, 0.68 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 845 |
| Protein Name / Synonyms | Calsequestrin-2 |
| Concentration (lot specific) | 0.68 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
FLJ26321 antibody, FLJ93514 antibody, PDIB2 antibody, CASQ2 antibody, calsequestrin-2 antibody, "calsequestrin, cardiac muscle isoform antibody", "calsequestrin 2, fast-twitch, cardiac muscle antibody", calsequestrin 2 (cardiac muscle) antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
46 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 124 and 388 (O14958) of Calsequestrin 2
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Calsequestrin-2 antibody detects Calsequestrin-2 protein at cytosol on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
Calsequestrin-2 antibody (107-10570) dilution: 1:500.
Sample: Paraffin-embedded mouse muscle.
Calsequestrin-2 antibody (107-10570) dilution: 1:500.
Calsequestrin 2 antibody detects Calsequestrin 2 protein by western blot analysis.
A. 30 µg HCT116 whole cell lysate/extract
10 % SDS-PAGE
Calsequestrin 2 antibody (107-10570) dilution: 1:1000
A. 30 µg HCT116 whole cell lysate/extract
10 % SDS-PAGE
Calsequestrin 2 antibody (107-10570) dilution: 1:1000
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft , using Calsequestrin-2(107-10570) antibody at 1:500 dilution.
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.