Rabbit Anti-CASP9 (Center) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P55211 |
| Gene Id | 842 |
| Gene Symbols | CASP9 |
| Protein Name / Synonyms | CASP9 (Center) |
| Clonality | Polyclonal |
| Preparation | This CASP9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 183-211 amino acids from the Central region of human CASP9. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, IF |
| Research Area | Apoptosis |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Caspase 9 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade.
Specificity
Other Names
CASP9; MCH6; Caspase-9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; ICE-like apoptotic protease 6; Caspase-9 subunit p35; Caspase-9 subunit p10
NCBI Accession #
NP_001220.2;NP_001264983.1;NP_127463.2
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
46281
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; IF: 1:10-50
 | CASP9 Antibody (Center) (Cat# 102-17737) western blot analysis in Hela cell line and mouse L929 tissue lysates (35ug/lane).This demonstrates the CASP9 antibody detected the CASP9 protein (arrow). |
 | Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CASP9 antibody (Center) (Cat# 102-17737), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Confocal immunofluorescent analysis of CASP9 Antibody (Center)(Cat# 102-17737) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.