Rabbit Anti-Human CBFA2T2 (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | O43439 |
| Gene Id | 9139 |
| Gene Symbols | CBFA2T2 |
| Protein Name / Synonyms | CBFA2T2 (N-term) |
| Clonality | Polyclonal |
| Preparation | This CBFA2T2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-29 amino acids from the N-terminal region of human CBFA2T2. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | IF, Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 (AML1) gene fused to the 3'-region of the CBFA2T1 (MTG8) gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. The protein encoded by this gene binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. Several transcript variants are thought to exist for this gene, but the full-length natures of only three have been described.
Specificity
Other Names
CBFA2T2; EHT; MTGR1; Protein CBFA2T2; ETO homologous on chromosome 20; MTG8-like protein; MTG8-related protein 1; Myeloid translocation-related protein 1; p85
NCBI Accession #
NP_001028171.1;NP_001034798.1;NP_005084.1
Other Accession #
NP_001028171.1; NP_005084.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
67133
Recommended Dilutions
IF: 1:10-50; WB: 1:1000
 | Fluorescent image of Hela cell stained with CBFA2T2 Antibody (N-term)(Cat# 102-12910).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CBFA2T2 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°).CBFA2T2 immunoreactivity is localized to Nucleus significantly. |
 | CBFA2T2 Antibody (N-term) (Cat# 102-12910) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the CBFA2T2 antibody detected the CBFA2T2 protein (arrow). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.