Rabbit Anti-Human CD14 (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P08571 |
| Gene Id | 929 |
| Gene Symbols | CD14 |
| Protein Name / Synonyms | CD14 (N-term) |
| Clonality | Polyclonal |
| Preparation | This CD14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-83 amino acids from the N-terminal region of human CD14. |
| Concentration (lot specific) | 2.000 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, IF, FC |
| Research Area | Cancer, Inflammation, Adaptive Immunity |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
CD14 is a surface protein preferentially expressed on monocytes/macrophages. It binds lipopolysaccharide binding protein and recently has been shown to bind apoptotic cells.
Specificity
Other Names
CD14; Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; CD_antigen=CD14; Monocyte differentiation antigen CD14, urinary form; Monocyte differentiation antigen CD14, membrane-bound form
NCBI Accession #
NP_000582.1;NP_001035110.1;NP_001167575.1;NP_001167576.1
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
40076
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; IF: 1:10-50; FC: 1:10-50
 | Western blot analysis of CD14(arrow) using rabbit polyclonal CD14 Antibody (N-term) (Cat# 102-16732). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD14 gene (Lane 2) (Origene Technologies). |
 | CD14 Antibody (N-term) (Cat# 102-16732) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the CD14 antibody detected the CD14 protein (arrow). |
 | Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CD14 antibody (N-term) (Cat# 102-16732), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Confocal immunofluorescent analysis of CD14 Antibody (N-term)(Cat# 102-16732) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue). |
 | CD14 Antibody (N-term) (Cat# 102-16732) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.
