CD74 antibody [N1N2], N-Terminus, 0.88 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 972 |
| Protein Name / Synonyms | CD74, N-term |
| Concentration (lot specific) | 0.88 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Research Area | Adaptive Immunity |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
DHLAG antibody, HLADG antibody, II antibody, Ia-GAMMA antibody, CD74 antibody, MHC HLA-DR gamma chain antibody, HLA-DR antigens-associated invariant chain antibody, HLA-DR-gamma antibody, p33 antibody, HLA class II histocompatibility antigen gamma chain antibody, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) antibody, gamma chain of class II antigens antibody, Ia-associated invariant chain antibody, CD74 molecule, major histocompatibility complex, class II invariant chain antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
34 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 1 and 58 of CD74 (Uniprot ID# P04233)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
CD74 antibody [N1N2], N-term detects CD74 protein by Western blot analysis.
A. 30 µg GL261 whole cell lysate/extract
12 % SDS-PAGE
CD74 antibody [N1N2], N-term (107-10917) dilution: 1:1000
A. 30 µg GL261 whole cell lysate/extract
12 % SDS-PAGE
CD74 antibody [N1N2], N-term (107-10917) dilution: 1:1000
CD74 antibody [N1N2], N-term detects CD74 protein by Western blot analysis.
A. 30 µg Rat2 whole cell lysate/extract
12 % SDS-PAGE
CD74 antibody [N1N2], N-term (107-10917) dilution: 1:1000
A. 30 µg Rat2 whole cell lysate/extract
12 % SDS-PAGE
CD74 antibody [N1N2], N-term (107-10917) dilution: 1:1000
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using CD74(107-10917) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: Raji
12% SDS PAGE
107-10917 diluted at 1:5000
A: Raji
12% SDS PAGE
107-10917 diluted at 1:5000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.