Rabbit Anti-Human CNOT8 (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q9UFF9 |
| Gene Id | 9337 |
| Gene Symbols | CNOT8 |
| Protein Name / Synonyms | CNOT8 (C-term) |
| Clonality | Polyclonal |
| Preparation | This CNOT8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 227-255 amino acids from the C-terminal region of human CNOT8. |
| Concentration (lot specific) | 0.406 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Ubiquitous transcription factor required for a diverse set of processes. The CCR4-NOT complex functions as general transcription regulation complex.
Specificity
Predicted Reactivity
Mouse
Other Names
CNOT8; CALIF; POP2; CCR4-NOT transcription complex subunit 8; CAF1-like protein; CAF2; CCR4-associated factor 8
NCBI Accession #
NP_004770.4
Other Accession #
Q9D8X5
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
33540
Recommended Dilutions
WB: 1:1000; IF: 1:10-50; IHC: 1:10-50; FC: 1:10-50
 | CNOT8 Antibody (C-term) (Cat# 102-16801) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the CNOT8 antibody detected the CNOT8 protein (arrow). |
 | Fluorescent confocal image of Hela cell stained with CNOT8 Antibody (C-term)(Cat# 102-16801).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CNOT8 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). CNOT8 immunoreactivity is localized to Cytoplasm significantly and Nucleus weakly. |
 | Formalin-fixed and paraffin-embedded human colon carcinoma with CNOT8 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Flow cytometric analysis of CEM cells using CNOT8 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.