COMMD7 antibody, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 149951 |
| Protein Name / Synonyms | COMMD7 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
C20orf92 antibody, FLJ14987 antibody, MGC33315 antibody, dJ1085F17.3 antibody, COMMD7 antibody, COMM domain-containing protein 7 antibody, COMM domain containing 7 antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
23 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 52 and 142 of COMMD7
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
COMMD7 antibody detects COMMD7 protein at nucleus and cytoplasm by immunofluorescent analysis.
Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: COMMD7 protein stained by COMMD7 antibody (107-11002) diluted at 1:500.
Blue: Hoechst 33342 staining.
Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: COMMD7 protein stained by COMMD7 antibody (107-11002) diluted at 1:500.
Blue: Hoechst 33342 staining.
Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using COMMD7(107-11002) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: H1299
12% SDS PAGE
107-11002 diluted at 1:1000
A: 293T
B: A431
C: H1299
12% SDS PAGE
107-11002 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.