Rabbit Anti-Human CT1 Antibody
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Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Specificity | Cross reactivity with mouse and rat CT-1 was not tested yet|Rabbit anti-Human Cardiotrophin-1 antibody detects specifically target derived from humanrecombinant protein as well as HepG2 cell lysates |
| Accession Number | Q16619 |
| Gene Id | 1489 |
| Gene Symbols | CTF1 |
| Clonality | Polyclonal |
| Immunogen | Immunogen was expressing Human CT-1 protein. This antibody was produced from a rabbit immunizedwith the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinitychromatography. |
| Immunogen Sequence | CGLYREWLSRTEGDLGQLLPGGSA |
| Recommended Applications | ELISA (recommended work dilution= 1:800, 000(at least detecting 12.5 ng/ml), Western Blotting (recommended work dilution= 1:1000-5000(at least)), Immunodetection Analysis: Representative blot from a previous lot. Lane 1.recombinant protein CT-1. Lane 2. HepG2 cell lysate. The membrane blot wasprobed with anti-CT-1 primary antibody(0.25?g/ml).Proteins were visualized using a goat anti-rabbitsecondary antibody conjugated to HRP andchemiluminescence detection system. Arrows indicatecellular CT-1 from human and mouse cells (24 kDa). |
| Antigen Catalog Number | 230-00008 |
| Reconstitution | Supplied as lyophilized and purified antibody originally containing PBS, without Preservative Stabilizers,liking Sodium Azide. The final concentration is indicated on the shipping vial. Please avoid freeze-thaw cycles.The antibody is stable for at least years from the data of receipt when stored at -20°C to -70°C.Reconstituted antibody (recommended with H20 1 mg/ml) can also be aliquoted and stored frozen at -20°C to-70°C in a manual defrost freezer for months without detectable loss activity. Upon reconstitution, theantibody can be stored at 4°C for 1 month. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Cardiotrophin-1 (CT-1) is a cardiac hypertrophic factor and belongs to the IL-6 cytokine family.The protein interacts with the glycoprotein 130 (gp130)/leukemia inhibitory factor receptor beta (LIFR)heterodimer. CT-1 deficiency causes increased motoneuron cell death in spinal cord and brainstemnuclei of mice. In addition, CT-1 enhances transcription factor NF kappa B DNA-binding activities andactivates phosphatidylinositol 3-kinase (PI-3 kinase) in cardiac myocytes. Studies have revealed CT-1 ishighly expressed in the heart, prostate, skeletal muscle, and ovary, and expressed at the lower levels inlung, kidney, pancreas, thymus, small intestine, and testis. CT-1 is highly secreted by myotubes, andpromotes the survival of cultured embryonic mouse and rat MNs. CT-1 is associated withpathophysiology of heart diseases, like hypertension, myocardial infarction, valvular heart disease, andcongestive heart failure.
Specificity
Rabbit anti-Human Cardiotrophin-1 antibody detects specifically target derived from humanrecombinant protein as well as HepG2 cell lysates. Cross reactivity with mouse and rat CT-1 was nottested yet.
Storage
Store at -20°C, if not intended for use within a month, keep it at 4°C and minimize freezing and thawingwhen use.
Related Products
Human Cardiotrophin-1 (CT-1) ELISA Kit For Serum, Plasma, Cell Culture Supernatant and Urine(Cat# ELH-CT1-001 ); Recombinant Human Cardiotrophin-1 (Cat# 230-00008)
References
- Arce V, et. al. (1998) Synergistic effects of Schwann- and muscle-derived factors on motoneuronsurvival involve GDNF and cardiotrophin-1 (CT-1). J Neurosci 18:1440-1448.
- Ronald W, et al. (2001). Cardiotrophin-1, a muscle-derived Cytokine, is required for the survival ofsubpopulations of developing motoneurons. J Neurosci. 21:1283-1291.
- Arce V, et al. (1999) Cardiotrophin-1 requires LIFRbeta to promote survival of mouse motoneuronspurified by a novel technique. J Neurosci Res 55:119-126.
Expiration:
12 months from the date of shipment when stored properly.
