Rabbit Anti-Cullin 2 Antibody, 100 µl
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | Q13617 |
| Gene Id | 8453 |
| Gene Symbols | CUL2 |
| Protein Name / Synonyms | Cullin-2, CUL-2, CUL2 |
| Clonality | Polyclonal |
| Recommended Applications | Western Blotting, IF, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin- conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF).
Other Information
Antigen Type
Synthetic Peptide
Images
WB ( 1:1000)
Western blot analysis of lysate from MDA-MB-231 cell line,using Cullin 2 Antibody(C0163). C0163 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug.
IF (1:100)
Immunofluorescence analysis of NIH/3T3 cells, using Cullin 2 antibody .
IHC (1:50-1:100)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Cullin 2 antibody .
WB (1:1000)
Western blot analysis of extracts from HepG2 cells (Lane 2), using Cullin 2 Antibody. The lane on the left is treated with systhesized peptide.
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.