Cytokeratin 20 antibody [N2C2], Internal, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 54474 |
| Protein Name / Synonyms | Cytokeratin 20, Internal |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
CD20 antibody, CK-20 antibody, CK20 antibody, K20 antibody, KRT21 antibody, MGC35423 antibody, KRT20 antibody, cytokeratin-20 antibody, keratin-20 antibody, cytokeratin 20 antibody, protein IT antibody, "keratin, type I cytoskeletal 20 antibody", keratin 20 antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
48 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 75 and 317 of Cytokeratin 20 (Uniprot ID# P35900)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cytokeratin 20(107-10929) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded HT29 xenograft, using Cytokeratin 20(107-10929) antibody at 1:100 dilution.
Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
107-10929 diluted at 1:3000
A: Hela
10% SDS PAGE
107-10929 diluted at 1:3000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.