DDB1 antibody, 0.81 mg/ml (100 ul)
Loading...
Loading...
Loading...
Loading...
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 1642 |
| Protein Name / Synonyms | DDB1 |
| Concentration (lot specific) | 0.81 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Immunoprecipitation, Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
DDBA antibody, UV-DDB1 antibody, XAP1 antibody, XPCE antibody, XPE antibody, XPE-BF antibody, DDB1 antibody, DNA damage-binding protein a antibody, XPE-binding factor antibody, UV-DDB 1 antibody, xeroderma pigmentosum group E-complementing protein antibody, UV-damaged DNA-binding protein 1 antibody, DNA damage-binding protein 1 antibody, XAP-1 antibody, HBV X-associated protein 1 antibody, DDB p127 subunit antibody, UV-damaged DNA-binding factor antibody, damage-specific DNA-binding protein 1 antibody, damage-specific DNA binding protein 1, 127kDa antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
127 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 749 and 1140 of DDB1 (Uniprot ID# Q16531)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Immunoprecipitation,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000, IP: 1:500-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
DDB1 antibody immunoprecipitates DDB1 protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 µg HeLa whole cell lysate/extract B. Control with 2.5 µg of preimmune rabbit IgG C. Immunoprecipitation of DDB1 protein by 2.5 µg of DDB1 antibody (107-10185) 7.5% SDS-PAGE The immunoprecipitated DDB1 protein was detected by DDB1 antibody (107-10185) diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
Sample (50 ug of whole cell lysate)
A: Mouse brain
5% SDS PAGE
107-10185 diluted at 1:5000
A: Mouse brain
5% SDS PAGE
107-10185 diluted at 1:5000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DDB1(107-10185) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft , using DDB1(107-10185) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: Hep G2
7.5% SDS PAGE
107-10185 diluted at 1:1000
A: Hep G2
7.5% SDS PAGE
107-10185 diluted at 1:1000
Sample (30 ug of whole cell lysate)
A:NIH-3T3
7.5% SDS PAGE
107-10185 diluted at 1:1000
A:NIH-3T3
7.5% SDS PAGE
107-10185 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.