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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Accession Number | P06730 |
| Gene Id | 1977 |
| Gene Symbols | EIF4E |
| Protein Name / Synonyms | EIF4E |
| Clonality | Monoclonal |
| Preparation | This EIF4E monoclonal antibody is generated from mouse immunized with EIF4E recombinant protein. |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
EIF4E recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
Specificity
Other Names
EIF4E; EIF4EL1; EIF4F; Eukaryotic translation initiation factor 4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein
NCBI Accession #
NP_001124150.1;NP_001124151.1;NP_001959.1
Other Accession #
NP_001959.1
Format
Type
Mouse Monoclonal Antibody (Mab)
Calculated Molecular Weight (Da)
25097
Recommended Dilutions
IF: 1:10-50; WB: 1:100-500
 | Fluorescent image of Hela cell stained with EIF4E Antibody(Cat# 102-10076/SG101020AF).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EIF4E primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). EIF4E immunoreactivity is localized to Cytoplasm significantly. |
 | EIF4E Antibody (Cat# 102-10076) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the EIF4E antibody detected the EIF4E protein (arrow). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.
