Rabbit Anti-Human ELP2 (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q6IA86 |
| Gene Id | 55250 |
| Gene Symbols | ELP2 |
| Protein Name / Synonyms | ELP2 (C-term) |
| Clonality | Polyclonal |
| Preparation | This ELP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 737-765 amino acids from the C-terminal region of human ELP2. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | IF, Western Blotting, FC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
ELP2 regulates the ligand-dependent activation of STAT3. The protein acts as subunit of the RNA polymerase II elongator complex, which is a histone acetyltransferase component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4.
Specificity
Other Names
ELP2; STATIP1; Elongator complex protein 2; SHINC-2; STAT3-interacting protein 1
NCBI Accession #
NP_001229804.1;NP_001229805.1;NP_001229806.1;NP_001229807.1;NP_001229808.1;NP_060725.1
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
92500
Recommended Dilutions
IF: 1:100; WB: 1:1000; FC: 1:10-50
 | Fluorescent confocal image of SY5Y cells stained with ELP2 (C-term) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with 102-15203 ELP2 (C-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). Note the highly specific localization of the ELP2 immunosignal mainly to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000134759). |
 | Western blot analysis of ELP2 antibody (C-term) (Cat# 102-15203) in NCI-H460 cell line lysates (35ug/lane). ELP2 (arrow) was detected using the purified Pab. |
 | Flow cytometric analysis of NCI-H292 cells using ELP2 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.