Synonyms
EPS8 antibody, epidermal growth factor receptor kinase substrate 8 antibody, epidermal growth factor receptor pathway substrate 8 antibody
Specificity
Format
Product Type
Primary antibody
Formulation
1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
92 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 761 and 822 of EPS8 (Uniprot ID# Q12929)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

EPS8 antibody [C3], C-term detects EPS8 protein at cytosol and nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
EPS8 antibody [C3], C-term (107-10199) dilution: 1:500.

Sample (30 ug of whole cell lysate)
A: H1299
B: Hela
7.5% SDS PAGE
EPS8 antibody
107-10199 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.