Rabbit Anti-Human ErbB2/HER2 Antibody
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Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Specificity | The antibody specifically detects phosphorylated protein derived from pancreatic carcinoma cell line lysate at various concentrations. In both ELISA and Western Blot, this antibody shows no crossreactivity with other unrelated protein. ErbB2-specific antibodies with no cross-reaction to ErbB3 or ErbB4. |
| Accession Number | NP_004439 |
| Clonality | Polyclonal |
| Immunogen | Immunogen was synthetic peptide derived from human ERBB2. This antibody was obtained from a rabbit immunized with the immunogen. The IgG fraction of immunized serum was purified by affinity chromatography. |
| Immunogen Sequence | CDETEY(P)HADGGK |
| Recommended Applications | ELISA (recommended work dilution= 1:1000-5000), Western Blotting( recommended work dilution= 2-10 µg/ml), Note: Other applications are not tested yet. Optimal dilutions should be determined by each laboratory for each application. |
| Reconstitution | Supplied as 200 µl of purified antibody in 10 mM sodium HEPES (pH 7.5),150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Each vial concentration is 100 µg/ µl. Store at 20°C. Do not aliquot the antibody. |
| Research Area | Cancer, HER/ErbB Signaling, Tyrosine Kinase Signaling |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
HER2/neu (also known as ErbB-2, ERBB2) stands for "Human Epidermal growth factor Receptor 2" and is a protein giving higher aggressiveness in breast cancers. It is a member of the ErbB protein family, more commonly known as the epidermal growth factor receptor family. HER2/neu has also been designated as CD340 and p185. The receptor tyrosine kinase ErbB2 plays a crucial role in tumorigenesis. Investigations shown that loss of Hsp90 binding correlates with enhanced ErbB2 kinase activity and its transactivating potential, concomitant with constitutively increased phosphorylation of Tyr877, located in the activation loop of the kinase domain. Furthermore, Tyr877 phosphorylation is mediated by Src and that it is necessary for the enhanced kinase activity of ErbB2.
Typical Data
Expiration
12 months from the date of shipment when stored properly.
