FAN antibody [N2C1], Internal, 0.93 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 8439 |
| Protein Name / Synonyms | FAN, Internal |
| Concentration (lot specific) | 0.93 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
FAN antibody, NSMAF antibody, protein FAN antibody, factor associated with N-SMase activation antibody, factor associated with neutral sphingomyelinase activation antibody, neutral sphingomyelinase (N-SMase) activation associated factor antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
104 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 262 and 570 of FAN (Uniprot ID# Q92636)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded T(gastric CA) , using NSMAF(107-10400) antibody at 1:100 dilution.
Western Blot analysis of NSMAF expression in transfected 293T cell line by NSMAF polyclonal antibody.
A: Non-transfected lysate.
B: NSMAF transfected lysate.
7.5% SDS PAGE
107-10400 diluted at 1:500
A: Non-transfected lysate.
B: NSMAF transfected lysate.
7.5% SDS PAGE
107-10400 diluted at 1:500
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.