GAD67 antibody, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 2571 |
| Protein Name / Synonyms | GAD67 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
FLJ45882 antibody, GAD antibody, SCP antibody, GAD1 antibody, GAD-67 antibody, glutamate decarboxylase 67 kDa isoform antibody, glutamate decarboxylase 1 antibody, 67 kDa glutamic acid decarboxylase antibody, glutamate decarboxylase 1 (brain, 67kDa) antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
67 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 5 and 216 of GAD67 (Uniprot ID# Q99259)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
GAD67 antibody detects GAD67 protein at on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
GAD67 antibody (107-10298) dilution: 1:500.
Sample: Paraffin-embedded mouse fore brain.
GAD67 antibody (107-10298) dilution: 1:500.
GAD67 antibody detects GAD67 protein by Western blot analysis.
A. 30 µg SK-N-SH whole cell lysate/extract
B. 30 µg SK-N-AS whole cell lysate/extract
7.5 % SDS-PAGE
GAD67 antibody (107-10298) dilution: 1:500
A. 30 µg SK-N-SH whole cell lysate/extract
B. 30 µg SK-N-AS whole cell lysate/extract
7.5 % SDS-PAGE
GAD67 antibody (107-10298) dilution: 1:500
GAD67 antibody detects GAD67 protein by Western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg whole cell lysate/extract of human GAD1-transfected 293T cells
7.5 % SDS-PAGE
GAD67 antibody (107-10298) dilution: 1:5000
A. 30 µg 293T whole cell lysate/extract
B. 30 µg whole cell lysate/extract of human GAD1-transfected 293T cells
7.5 % SDS-PAGE
GAD67 antibody (107-10298) dilution: 1:5000
GAD67 antibody detects GAD67 protein by Western blot analysis.
A. 50 µg mouse brian lysate/extract
7.5 % SDS-PAGE
GAD67 antibody (107-10298) dilution: 1:500
A. 50 µg mouse brian lysate/extract
7.5 % SDS-PAGE
GAD67 antibody (107-10298) dilution: 1:500
GAD67 antibody detects GAD67 protein by Western blot analysis.
A. 50 µg rat brain lysate/extract
7.5 % SDS-PAGE
GAD67 antibody (107-10298) dilution: 1:500
A. 50 µg rat brain lysate/extract
7.5 % SDS-PAGE
GAD67 antibody (107-10298) dilution: 1:500
GAD67 antibody detects GAD67 protein by immunofluorescent analysis.
Sample: GAD67-transfected (right) or untransfected (left) 293T cells were fixed in 4% paraformaldehyde for 15 min.
Green: GAD67 protein stained by GAD67 antibody (107-10298) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 ?m.
Sample: GAD67-transfected (right) or untransfected (left) 293T cells were fixed in 4% paraformaldehyde for 15 min.
Green: GAD67 protein stained by GAD67 antibody (107-10298) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 ?m.
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.