Product Description

Specifications

Size	20 µg, 100 µg, 200 µg, 500 µg
Estimated Lead Time	1-2 business days
Species	Human
Accession Number	NP_002045
Clonality	Polyclonal
Immunogen	Immunogen was synthetic peptide derived from Human GLP-1. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinity chromatography.
Immunogen Sequence	CSSYLEGQAAKEFIAWLVKGRG
Recommended Applications	ELISA (recommended work dilution= 1: 4, 000(at least detecting 250ng/ml))
Reconstitution	Supplied as lyophilized and purified antibody originally containing PBS, without Preservative Stabilizers, such as Sodium Azide. The final concentration is indicated on the shipping vial. Please avoid freeze-thaw cycles. The antibody is stable for at least years from the data of receipt when stored at -20°C to -70°C. Reconstituted antibody (recommended with H₂0 1 mg/ml) can also be aliquoted and stored frozen at -20°C to -70°C in a manual defrost freezer for months without detectable loss activity. Upon reconstitution, the antibody can be stored at 4°C for 1 month.
Shipping Type	Ambient temperature
Storage	-20°C

Description

The proglucagon gene is expressed in both pancreatic A cells and intestinal L cells. Tissue-specific post-translational processing of proglucagon by the prohormone convertase produces the different proglucagon derived peptides (PGDPs) in both pancreas and intestine. The most notable pancreatic PGDP is glucagon, whereas the L cells produce several structurally related peptides, including glucagon-like peptide (GLP)-1 and GLP-2, as well as glicentin and oxyntomodulin, which contain glucagon sequence in their molecules. Among PGDPs, GLP-2 has recently been found to show intestinal epithelial proliferation. GLP-1 has a major effect of enhancing the release of insulin in response to a glucose stimulus, and coincidentally, suppressing secretion of glucagon. GLP-1 has been shown to potently inhibit several aspects of digestive function, including gastric emptying, gastric secretion and pancreatic secretion.

Specificity

The rabbit anti-human Glucagon-like peptide-1 antibody detected specifically target derived from human recombinant protein and cellular lysates. Cross reactivity with mouse and rat GLP-1 has not yet been tested.

Storage

Store at -20°C, if not intended for use within a month, keep it at 4°C and minimize freezing and thawing when use.

References

Tang-Christensen, M and et al., Glucagon-like peptide 1(7-36) amide's central inhibition of feeding and peripheral inhibition of drinking are abolished by neonatal monosodium glutamate treatment. Diabetes47, 530537 (1998).
McMahon, L.R. & Wellman, P.J. PVN infusion of GLP-1-(736) amide suppresses feeding but does not induce aversion or alter locomotion in rats. Am. J. Physiol. 274, R23R29 (1998). Turton, M.D. et al. A role for glucagon-like peptide-1 in the central regulation of feeding. Nature 379, 6972 (1996).

Expiration:

12 months from the date of shipment when stored properly.

Description

Specificity

Storage

Store at -20°C, if not intended for use within a month, keep it at 4°C and minimize freezing and thawing when use.

References

Tang-Christensen, M and et al., Glucagon-like peptide 1(7-36) amide's central inhibition of feeding and peripheral inhibition of drinking are abolished by neonatal monosodium glutamate treatment. Diabetes47, 530537 (1998).
McMahon, L.R. & Wellman, P.J. PVN infusion of GLP-1-(736) amide suppresses feeding but does not induce aversion or alter locomotion in rats. Am. J. Physiol. 274, R23R29 (1998). Turton, M.D. et al. A role for glucagon-like peptide-1 in the central regulation of feeding. Nature 379, 6972 (1996).

Expiration:

12 months from the date of shipment when stored properly.

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