Mouse Anti-Human H2AFX (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Accession Number | P16104 |
| Gene Id | 3014 |
| Gene Symbols | H2AFX |
| Protein Name / Synonyms | H2AFX (N-term) |
| Clonality | Monoclonal |
| Preparation | This H2AFX antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human H2AFX. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IHC-P |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
Specificity
Predicted Reactivity
Mouse, Zebrafish
Other Names
H2AFX;H2AX; Histone H2A.x
NCBI Accession #
NP_002096.1
Other Accession #
P27661; Q7ZUY3
Format
Type
Mouse Monoclonal Antibody (Mab)
Calculated Molecular Weight (Da)
15145
Recommended Dilutions
WB: 1:1000
 | H2AFX Antibody (N-term) (Cat# 102-10421) western blot analysis in CEM,HepG2,Raji cell line lysates (35ug/lane).This demonstrates the H2AFX antibody detected the H2AFX protein (arrow). |
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.