Rabbit Anti-HDAC1 (Center S423) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q13547 |
| Gene Id | 3065 |
| Gene Symbols | HDAC1 |
| Protein Name / Synonyms | HDAC1 (Center S423) |
| Clonality | Polyclonal |
| Preparation | This HDAC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 402-430 amino acids from the Central region of human HDAC1. |
| Concentration (lot specific) | 0.450 mg/ml |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.
Specificity
Other Names
HDAC1; RPD3L1; Histone deacetylase 1
NCBI Accession #
NP_004955.2
Other Accession #
NP_004955.2
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
55103
Recommended Dilutions
WB: 1:1000; IF: 1:10-50
 | HDAC1 Antibody (S423) (Cat# 102-13174) western blot analysis in Hela cell line and mouse testis,rat testis tissue lysates (35ug/lane).This demonstrates the HDAC1 antibody detected the HDAC1 protein (arrow). |
 | Fluorescent confocal image of Hela cell stained with HDAC1 Antibody (Center S423)(Cat# 102-13174).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HDAC1 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). HDAC1 immunoreactivity is localized to Nucleus significantly. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.