HDAC4 Polyclonal Antibody
Loading...
Loading...
Loading...
Loading...
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P56524 |
| Gene Id | 9759 |
| Gene Symbols | HDAC4 |
| Protein Name / Synonyms | HDAC4, AHO3, BDMR, HA6116, HD4, HDAC-4, HDAC-A, HDACA, histone deacetylase 4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF, IP |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3.
Dilutions
WB 1:500 - 1:2000
IF 1:50 - 1:200
IP 1:50 - 1:100
IF 1:50 - 1:200
IP 1:50 - 1:100
Format
Calculated MW
106kDa/119kDa
Observed MW
117kDa
Immunogen
A synthetic peptide of human HDAC4
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using HDAC4 antibody (144-00239) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of A549 cells using HDAC4 antibody (144-00239). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug HDAC4 antibody (144-00239). Western blot was performed from the immunoprecipitate using HDAC4 antibody (144-00239) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.