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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q9BY41 |
| Gene Id | 55869 |
| Gene Symbols | HDAC8 |
| Protein Name / Synonyms | HDAC8, CDA07, CDLS5, HD8, HDACL1, MRXS6, RPD3, WTS, histone deacetylase 8 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase family. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. Multiple transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
15kDa/16kDa/17kDa/28kDa/29kDa/31kDa/41kDa
Observed MW
42kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-377 of human HDAC8 (NP_060956.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using HDAC8 Antibody (144-05829) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.![Western blot - [KO Validated] HDAC8 Polyclonal Antibody (144-05829)](https://cdn.sanity.io/images/g51potcz/production/3289b69d2baf49afe871bfe3cdf6da6fccbdcbe3-188x350.jpg)
Western blot analysis of extracts from normal (control) and HDAC8 knockout (KO) HeLa cells, using HDAC8 antibody (144-05829) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.![Western blot - [KO Validated] HDAC8 Polyclonal Antibody (144-05829)](https://cdn.sanity.io/images/g51potcz/production/7c2344d9e846675a765fc63eab24d80708be1c6b-467x350.jpg)
Immunohistochemistry of paraffin-embedded rat testis using HDAC8 antibody (144-05829) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] HDAC8 Polyclonal Antibody (144-05829)](https://cdn.sanity.io/images/g51potcz/production/f131efda1eaa1f1cc4c988d599cecb04c84273ce-467x350.jpg)
Immunohistochemistry of paraffin-embedded rat spinal cord using HDAC8 antibody (144-05829) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] HDAC8 Polyclonal Antibody (144-05829)](https://cdn.sanity.io/images/g51potcz/production/8561e5794faee3a0882e7e2ea7d4f86eb28da552-467x350.jpg)
Immunohistochemistry of paraffin-embedded human breast cancer using HDAC8 antibody (144-05829) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] HDAC8 Polyclonal Antibody (144-05829)](https://www.raybiotech.com/files/images/Antibodies/144/144-05829/5.jpg)
Expiration:
12 months from the date of shipment when stored properly.