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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P68431 |
| Gene Id | 8350 |
| Gene Symbols | HIST1H3A |
| Protein Name / Synonyms | HIST1H3A, H3/A, H3FA, histone H3.1 |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
15kDa
Observed MW
17kDa
Immunogen
A synthetic phosphorylated peptide around T3 of human HIST1H3A (NP_003520.1).
Modification
Phospho T3
Storage
-20°C
Images
Western blot analysis of extracts of C6 cells, using Phospho-HIST1H3A-T3 pAb (144-61551).
C6 cells were treated by Nocodazole (50ng/ml) for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western blot analysis of extracts of various cell lines, using Phospho-HIST1H3A-T3 pAb (144-61551).
293T cells were treated by Nocodazole (50ng/ml) for 20 hours.RAW264.7 cells were treated by Nocodazole (50ng/ml) for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 1s.
Expiration:
12 months from the date of shipment when stored properly.