Rabbit Anti-Human HNRNPD (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q14103 |
| Gene Id | 3184 |
| Gene Symbols | HNRNPD |
| Protein Name / Synonyms | HNRNPD (N-term) |
| Clonality | Polyclonal |
| Preparation | This HNRNPD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57-85 amino acids from the N-terminal region of human HNRNPD. |
| Concentration (lot specific) | 0.500 mg/ml |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are nucleic acid binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. This protein is implicated in the regulation of mRNA stability. Alternative splicing of this gene results in four transcript variants.
Specificity
Predicted Reactivity
Mouse, Rat
Other Names
HNRNPD; AUF1; HNRPD; Heterogeneous nuclear ribonucleoprotein D0; AU-rich element RNA-binding protein 1
NCBI Accession #
NP_001003810.1;NP_002129.2;NP_112737.1;NP_112738.1
Other Accession #
Q9JJ54; Q60668; NP_002129.2; NP_112738.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
38434
Recommended Dilutions
WB: 1:1000; IF: 1:10-50
 | HNRNPD Antibody (N-term) (Cat# 102-12849) western blot analysis in 293T,A431,NCI-H292,Raji cell line lysates (35ug/lane).This demonstrates the HNRNPD antibody detected the HNRNPD protein (arrow). |
 | Fluorescent confocal image of Hela cell stained with HNRNPD Antibody (N-term)(Cat# 102-12849).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HNRNPD primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). HNRNPD immunoreactivity is localized to Nucleus and Cytoplasm significantly. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.
