Rabbit Anti-IL4 (C-term) Antibody, 400 ul
Loading...
Loading...
Loading...
Loading...
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P05112 |
| Gene Id | 3565 |
| Gene Symbols | IL4 |
| Protein Name / Synonyms | IL4 (C-term) |
| Clonality | Polyclonal |
| Preparation | This L4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122-150 amino acids from the C-terminal region of human L4. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Research Area | Cancer, Inflammation, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
IL4 is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome.
Specificity
Predicted Reactivity
Monkey
Other Names
IL4; Interleukin-4; B-cell stimulatory factor 1; Binetrakin; Lymphocyte stimulatory factor 1; Pitrakinra
NCBI Accession #
NP_000580.1;NP_758858.1
Other Accession #
P79339
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
17492
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; FC: 1:10-50
 | (LEFT)Western blot analysis of IL4 Antibody (C-term) (Cat# 102-16447) in WiDr cell line lysates (35ug/lane).IL4 (arrow) was detected using the purified Pab.(RIGHT)Western blot analysis of IL4 Antibody (C-term) (Cat# 102-16447) in mouse cerebellum cell line lysates (35ug/lane).IL4(arrow) was detected using the purified Pab. |
 | IL4 Antibody (C-term) (Cat# 102-16447) IHC analysis in formalin fixed and paraffin embedded tonsil followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IL4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
 | IL4 Antibody (C-term) (Cat# 102-16447) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.