Rabbit Anti-Human IL5RA (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q01344 |
| Gene Id | 3568 |
| Gene Symbols | IL5RA |
| Protein Name / Synonyms | IL5RA (N-term) |
| Clonality | Polyclonal |
| Preparation | This IL5RA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-76 amino acids from the N-terminal region of human IL5RA. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
IL5RA is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4.
Specificity
Other Names
IL5RA; IL5R; Interleukin-5 receptor subunit alpha; CDw125; CD_antigen=CD125; Flags: Precursor
NCBI Accession #
NP_000555.2;NP_001230028.1;NP_783851.1;NP_783852.1;NP_783853.1;NP_783854.1;NP_783855.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
47685
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50
 | Western blot analysis of lysate from MDA-MB-453 cell line, using IL5RA Antibody (N-term)(Cat# 102-16445). 102-16445 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane. |
 | IL5RA Antibody (N-term) (Cat# 102-16445) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IL5RA Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
 | IL5RA Antibody (N-term) (Cat# 102-16445) flow cytometric analysis of U251 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.