Rabbit Anti-Human ILK1/ILK2 (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | O55222 |
| Gene Id | 16202 |
| Gene Symbols | ILK |
| Protein Name / Synonyms | ILK1/ILK2 (N-term) |
| Clonality | Polyclonal |
| Preparation | This ILK1/ILK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 11-41 amino acids from the N-terminal region of human ILK1/ILK2. |
| Concentration (lot specific) | 1.430 mg/ml |
| Recommended Applications | Western Blotting, IHC-P |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. ILK encodes a predicted 451-amino acid protein, with an apparent molecular weight of 59 kD. The ILK protein is a serine/threonine protein kinase with 4 ankyrin-like repeats. ILK regulates integrin-mediated signal transduction.
Specificity
Predicted Reactivity
Rat, Bovine
Other Names
Ilk; Integrin-linked protein kinase
NCBI Accession #
NP_001155196.1;NP_034692.2
Other Accession #
Q99J82; Q13418; Q3SWY2
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
51373
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100
 | Western blot analysis of anti-ILK1 Pab (Cat# 102-17517) in HL60 cell lysate. ILK1 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. |
 | Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
 | Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ILK1/ILK2 Antibody (N-term) (Cat# 102-17517), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
Antigen Source
MOUSE
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.