Rabbit Anti-Human Interferon-inducible protein (IFITM3) (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q01628 |
| Gene Id | 10410 |
| Gene Symbols | IFITM3 |
| Protein Name / Synonyms | Interferon-inducible protein (IFITM3) (N-term) |
| Clonality | Polyclonal |
| Preparation | This Interferon-inducible protein (IFITM3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human Interferon-inducible protein (IFITM3). |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
The family of interferon-induced transmembrane protein (Ifitm/mil/fragilis) cell surface proteins may modulate cell adhesion and influence cell differentiation.
Specificity
Other Names
IFITM3; Interferon-induced transmembrane protein 3; Dispanin subfamily A member 2b; Interferon-inducible protein 1-8U
NCBI Accession #
NP_066362.2
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
14632
Recommended Dilutions
WB: 1:1000; IF: 1:100-500
 | Western blot analysis of lysate from HT1080 cell line,using IFITM3 Antibody (N-term)(Cat# 102-11135).102-11135 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane. |
 | IFITM3 Antibody (N-term) (Cat# 102-11135) western blot analysis in Hela,293,A549,NCI-H460,HepG2,Sk-Br-3,A375 cell line lysates (35ug/lane).This demonstrates the IFITM3 antibody detected the IFITM3 protein (arrow). |
 | Fluorescent confocal image of HeLa cells stained with IFITM3 (N-term) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with Fragilis (IFITM3) (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (5.25 µM, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 3 min). Note the highly specific localization of the IFITM3 immunoreactivity to the Golgi. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.
