Rabbit Anti-MEK2 (MAP2K2) (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P36507 |
| Gene Id | 5605 |
| Gene Symbols | MAP2K2 |
| Protein Name / Synonyms | MEK2 (MAP2K2) (N-term) |
| Clonality | Polyclonal |
| Preparation | This MEK2 (MAP2K2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human MEK2 (MAP2K2). |
| Concentration (lot specific) | 0.500 mg/ml |
| Recommended Applications | Western Blotting, IF |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
MAP2K2 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. The inhibition or degradation of this kinase is found to be involved in the pathogenesis of Yersinia and anthrax.
Specificity
Other Names
MAP2K2; MEK2; MKK2; PRKMK2; Dual specificity mitogen-activated protein kinase kinase 2; ERK activator kinase 2; MAPK/ERK kinase 2
NCBI Accession #
NP_109587.1
Other Accession #
P36506; Q63932
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
44424
Recommended Dilutions
WB: 1:1000; IF: 1:10-50
 | MEK2 (MAP2K2) Antibody (N-term) (Cat# 102-17728) western blot analysis in Hela,MCF-7,rat C6 cell line and mouse Liver,rat Brain tissue lysates (35ug/lane).This demonstrates the MEK2 (MAP2K2) antibody detected the MEK2 (MAP2K2) protein (arrow). |
 | Fluorescent confocal image of U251 cell stained with MEK2 (MAP2K2) Antibody (N-term)(Cat# 102-17728).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MEK2 (MAP2K2) primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).MEK2 (MAP2K2) immunoreactivity is localized to Cytoplasm significantly. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.