Rabbit Anti-Human NFKBIA ( S32/36 ) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P25963 |
| Gene Id | 4792 |
| Gene Symbols | NFKBIA |
| Protein Name / Synonyms | NFKBIA ( S32/36 ) |
| Clonality | Polyclonal |
| Preparation | This NFKBIA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-41 amino acids from human NFKBIA. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC, IF |
| Research Area | Cancer, Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).
Specificity
Predicted Reactivity
Mouse, Pig
Other Names
NFKBIA; IKBA; MAD3; NFKBI; NF-kappa-B inhibitor alpha; I-kappa-B-alpha; Major histocompatibility complex enhancer-binding protein MAD3
NCBI Accession #
NP_065390.1
Other Accession #
Q08353; Q9Z1E3
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
35609
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50; IF: 1:10-50
 | Western blot analysis of NFKBIA-S32/36 (Cat# 102-17741) in Hela cell line lysates (35ug/lane). NFKBIA (arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human hepatocarcinoma with NFKBIA Antibody (S32/36 ), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Flow cytometric analysis of hela cells using NFKBIA Antibody ( S32/36 )(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
 | Confocal immunofluorescent analysis of NFKBIA Antibody ( S32/36 )(Cat# 102-17741) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.