Rabbit Anti-Human NFKBp65 (C-term S536) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q04206 |
| Gene Id | 5970 |
| Gene Symbols | RELA |
| Protein Name / Synonyms | NFKBp65 (C-term S536) |
| Clonality | Polyclonal |
| Preparation | This NFKBp65 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 517-539 amino acids from the C-terminal region of human NFKBp65. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | IF, Western Blotting |
| Research Area | Angiogenesis, Cancer, Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).
Specificity
Predicted Reactivity
Mouse, Pig
Other Names
RELA; NFKB3; Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
NCBI Accession #
NP_001138610.1;NP_001230913.1;NP_068810.3
Other Accession #
Q04207; Q6QN13; P49895; NP_001138610.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
60219
Recommended Dilutions
IF: 1:10-50; WB: 1:1000
 | Fluorescent image of U251 cell stained with NFKBp65 Antibody(C-termS536)(Cat# 102-13731).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFKBp65 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°).NFKBp65 immunoreactivity is localized to Cytoplasm significantly. |
 | NFKBp65 Antibody (pS536) (Cat# 102-13731) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the NFKBp65 antibody detected the NFKBp65 protein (arrow). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.