NLK antibody [N3C3], 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 51701 |
| Protein Name / Synonyms | NLK |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
DKFZp761G1211 antibody, FLJ21033 antibody, NLK antibody, serine/threonine-protein kinase NLK antibody, nemo-like kinase antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
58 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 234 and 527 of NLK (Uniprot ID# Q9UBE8)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000, IHC-P: 1-100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
107-10717 diluted at 1:1000
A: Mouse brain
7.5% SDS PAGE
107-10717 diluted at 1:1000
NLK antibody [N3C3] detects NLK protein at cytosol and nucleus on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
NLK antibody [N3C3] (107-10717) dilution: 1:500.
Sample: Paraffin-embedded mouse ovary.
NLK antibody [N3C3] (107-10717) dilution: 1:500.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NLK(107-10717) antibody at 1:100 dilution.
Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
NLK antibody
107-10717 diluted at 1:1000
A: Hela
7.5% SDS PAGE
NLK antibody
107-10717 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.