Rabbit Anti-Human NPM1 (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P06748 |
| Gene Id | 4869 |
| Gene Symbols | NPM1 |
| Protein Name / Synonyms | NPM1 (C-term) |
| Clonality | Polyclonal |
| Preparation | This NPM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 198-226 amino acids from the C-terminal region of human NPM1. |
| Concentration (lot specific) | 2.000 mg/ml |
| Recommended Applications | Western Blotting, FC, IHC-P, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
NPM1 is a ubiquitously expressed nucleolar protein that shuttles between the nucleus and cytoplasm. It is implicated in multiple functions, including ribosomal protein assembly and transport, control of centrosome duplication, and regulation of the tumor suppressor ARF. NPM1 mutations that relocalize NPM1 from the nucleus into the cytoplasm are associated with development of acute myeloid leukemia.
Specificity
Other Names
NPM1; NPM; Nucleophosmin; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Numatrin
NCBI Accession #
NP_001032827.1;NP_002511.1;NP_954654.1
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
32575
Recommended Dilutions
IF: 1:10-50; WB: 1:1000; FC: 1:10-50; IHC: 1:10-50
 | Fluorescent image of A549 cell stained with NPM1 Antibody (C-term)(Cat# 102-15171/SH090717BE).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NPM1 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°).NPM1 immunoreactivity is localized to Nucleus and Nucleolus significantly. |
 | NPM1 Antibody (C-term) (Cat# 102-15171) western blot analysis in Hela,Jurkat cell line lysates (35ug/lane).This demonstrates the NPM1 antibody detected the NPM1 protein (arrow). |
 | NPM1 Antibody (C-term) (Cat# 102-15171) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
 | NPM1 Antibody (C-term) (102-15171)immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NPM1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.