Rabbit Anti-PHB2 (Y248) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | Q99623 |
| Gene Id | 11331 |
| Gene Symbols | PHB2 |
| Protein Name / Synonyms | PHB2 (Y248) |
| Clonality | Polyclonal |
| Preparation | This PHB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 225-255 amino acids from human PHB2. |
| Concentration (lot specific) | 0.440 mg/ml |
| Recommended Applications | Western Blotting, IHC-P |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
PHB2 acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases. It functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. It competes with NCOA1 for modulation of ER transcriptional activity and is probably involved in regulating mitochondrial respiration activity and in aging.
Specificity
Predicted Reactivity
Rat, Bovine
Other Names
PHB2; BAP; REA; Prohibitin-2; B-cell receptor-associated protein BAP37; D-prohibitin; Repressor of estrogen receptor activity
NCBI Accession #
NP_001138303.1;NP_001254629.1
Other Accession #
Q5XIH7; O35129; Q2HJ97; NP_009204
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
33296
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100
 | Western blot analysis of PHB2-Y248(Cat# 102-17234) in MDA-MB435 cell line lysates (35ug/lane).PHB2 (arrow) was detected using the purified Pab. |
 | Western blot analysis of PHB2-Y248 (Cat# 102-17234) in mouse kidney tissue lysates (35ug/lane). PHB2 (arrow) was detected using the purified Pab. |
 | Western blot analysis of PHB2 (arrow) using PHB2 Antibody (Y248) (Cat# 102-17234). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PHB2 gene (Lane 2) (Origene Technologies). |
 | PHB2 Antibody (Y248)(Cat# 102-17234) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PHB2 Antibody (Y248) for immunohistochemistry. Clinical relevance has not been evaluated. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.