Synonyms
GSD9A antibody, MGC133071 antibody, PHK antibody, PYK antibody, PYKL antibody, XLG antibody, XLG2 antibody, PHKA2 antibody, phosphorylase kinase alpha-subunit antibody, "phosphorylase b kinase regulatory subunit alpha, liver isoform antibody", phosphorylase b kinase regulatory subunit alpha liver isoform antibody, phosphorylase kinase alpha L subunit antibody, "phosphorylase kinase, alpha 2 (liver) antibody"
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
138 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 1172 and 1235 of PHKA2 (Uniprot ID# P46019)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded Colon ca, using PHKA2(107-10757) antibody at 1:250 dilution.

Sample (30 ug of whole cell lysate)
A: Hep G2
5% SDS PAGE
107-10757 diluted at 1:500
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.