Phospho-PKD/PKCmu-S744/748 pAb
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q15139 |
| Gene Id | 5587 |
| Gene Symbols | PRKD1 |
| Protein Name / Synonyms | PRKD1, CHDED, PKC-MU, PKCM, PKD, PRKCM, protein kinase D1 |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Research Area | Angiogenesis, Cardiovascular Disease, Inflammation, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is a serine/threonine protein kinase involved in many cellular processes, including Golgi body membrane integrity and transport, cell migration and differentiation, MAPK8/JNK1 and Ras pathway signaling, MAPK1/3 (ERK1/2) pathway signaling, cell survival, and regulation of cell shape and adhesion.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
101kDa
Observed MW
102kDa
Immunogen
A phospho specific peptide corresponding to residues surrounding S744/748 of human PKD/PKCmu
Modification
Phospho S744/748
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using Phospho-PKD/PKCmu-S744/748 antibody (144-61503) at 1:1000 dilution. NIH/3T3 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight or treated by PDGF (100ng/ml) for 30 minutes after serum-starvation overnight.C6 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight or treated by PDGF (50 ng/ml) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 5s.
Expiration:
12 months from the date of shipment when stored properly.