Mouse Anti-Human PTEN Antibody
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Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Specificity | This antibody was selected for its ability to detect human PTEN. In ELISA and Western Blot, this antibody showed specifically binding to human recombinant protein, and there was cross-reactivity with tested proteins from other species. |
| Accession Number | P60484 |
| Gene Id | 5728 |
| Gene Symbols | PTEN MMAC1 TEP1 |
| Clonality | Monoclonal |
| Immunogen | Immunogen was recombinant protein derived from human PTEN. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G/A affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= 1: 1, 000-5, 000), Western Blotting (recommended work dilution= 1: 1, 000-2, 000), Immunohistology -paraffin (recommended work dilution= 1:50-100) |
| Antigen Catalog Number | 230-10071 |
| Reconstitution | Supplied as lyophilized and purified antibody originally containing PBS, without Preservative Stabilizers, such as Sodium Azide. The final concentration is indicated on the shipping vial. The antibody is stable for at least years from the data of receipt when stored at -20°C to -70°C. Reconstituted antibody (recommended with H20 1 mg/ml) can also be aliquoted and stored frozen at -20°C to -70°C in a manual defrost freezer for months without detectable loss activity. Upon reconstitution, the antibody can be stored at 4°C for 1 month. Please avoid freeze-thaw cycles. |
| Research Area | Cardiovascular Disease, Cancer, Akt Signaling |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
PTEN, phosphatase and tensin homolog (TEP1), is a dual specificity protein/3-lipid phosphatase that functions as a tumor suppressor and negative growth regulator. Inactivation of PTEN is associated with a variety cancers including glioblastoma, melanoma, and prostate, breast, and endometrial cancers. The PTEN gene product is a putative protein tyrosine phosphatase that is localized to the cytoplasm and shares extensive homology with the cytoskeletal proteins tensin and auxilin.
Typical Data
Storage/Stability
Store at -20°C, if not intended for use within a month, keep it at 4°C and minimize freezing and thawing when use.
Expiration
12 months from the date of shipment when stored properly.
References
- Steck, P.A., et al. 1997. Identification of a candidate tumour suppressor gene, MMAC1, at chromosome 10q23.3 that is mutated in multiple advanced cancers. Nat. Genet. 15: 356-362.
- Li, J., et al. 1997. PTEN, a putative protein tyrosine phosphatase gene mutated in human brain, breast, and prostate cancer. Science 275:1943-1947.
