Rabbit Anti-Rat TNF-alpha
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Anti-Rat TNF-alpha Antibody
| Size | 0.1 mg |
| Estimated Lead Time | 1-2 weeks |
| Species | Rat |
| Specificity | This product recognizes rat tumour necrosis factor alpha (TNF alpha). Rat TNF-alpha is a 17kD cytokine which is produced by many cell types including macrophages, monocytes, neutrophils and a population of activated NK cells. |
| Host Species | Rabbit |
| Protein Name / Synonyms | TNF-alpha / TNF |
| Preparation | Antisera to rat TNF alpha were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. |
| Concentration (lot specific) | IgG concentration 1.0 mg/ml after reconstitution. |
| Recommended Applications | ELISA (dilution: 0.1, 0.2 ug/ml), Functional Assays, Immunohistology - Paraffin, Western Blotting |
| Reconstitution | Reconstitute with 0.1ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. N.B. For functional studies do not add sodium azide |
| Research Area | Cardiovascular Disease, Inflammation |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Prior to reconstitution store at +4 °C. Following reconstitution store at -20 °C.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.
Antisera to rat TNF alpha were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Anti-Rat TNF-alpha Antibody