RPA14 antibody, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 6119 |
| Protein Name / Synonyms | RPA14 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
REPA3 antibody, RPA3 antibody, RF-A protein 3 antibody, RP-A p14 antibody, replication protein A 14 kDa subunit antibody, replication factor A protein 3 antibody, replication protein A3, 14kDa antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
14 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 121 of RPA14 (Uniprot ID# P35244)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
RPA14 antibody detects RPA14 protein by Western blot analysis.
A. 30 µg 293T whole cell lysate/extract
15 % SDS-PAGE
RPA14 antibody (107-10180) dilution: 1:1000
A. 30 µg 293T whole cell lysate/extract
15 % SDS-PAGE
RPA14 antibody (107-10180) dilution: 1:1000
Immunohistochemical analysis of paraffin-embedded lung CA patient tumor, using RPA 14 kDa subunit (107-10180) antibody at 1:100 dilution.
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.