Synonyms
FTE1 antibody, MFTL antibody, MGC23240 antibody, RPS3A antibody, 40S ribosomal protein S3a antibody, v-fos transformation effector protein 1 antibody, fte-1 antibody, ribosomal protein S3A antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
30 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 14 and 264 of RPS3A (Uniprot ID# P61247)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

RPS3A antibody detects RPS3A protein at cytosol on mouse brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain stem.
RPS3A antibody (107-10505) dilution: 1:200.

RPS3A antibody detects RPS3A protein by Western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
C. 30 µg C8D30 whole cell lysate/extract
D. 30 µg NIH-3T3 whole cell lysate/extract
E. 30 µg BCL-1 whole cell lysate/extract
F. 30 µg Raw264.7 whole cell lysate/extract
G. 30 µg C2C12 whole cell lysate/extract
12 % SDS-PAGE
RPS3A antibody (107-10505) dilution: 1:1000

RPS3A antibody detects RPS3A protein by Western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
12 % SDS-PAGE
RPS3A antibody (107-10505) dilution: 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ribosomal protein S3a(107-10505) antibody at 1:200 dilution.

Sample(30 ug whole cell lysate)
A: 293T
B: A431
C: H1299
D: HeLa S3
E: Hep G2
F: MOLT4
G: Raji
12% SDS PAGE
107-10505 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.