SAP130 antibody [C3], C-Terminus, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 23450 |
| Protein Name / Synonyms | SAP130, C-term |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
KIAA0017 antibody, RSE1 antibody, SAP130 antibody, SF3b130 antibody, STAF130 antibody, SF3B3 antibody, "pre-mRNA splicing factor SF3b, 130 kDa subunit antibody", splicing factor 3B subunit 3 antibody, pre-mRNA-splicing factor SF3b 130 kDa subunit antibody, spliceosome-associated protein 130 antibody, SAP 130 antibody, "splicing factor 3b, subunit 3, 130kDa antibody"
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
136 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 1154 and 1217 of SAP130 (Uniprot ID# Q15393)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000[suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of methanol-fixed HeLa, using SAP130(107-10624) antibody at 1:500 dilution.
SAP130 antibody [C3], C-term detects SAP130 protein by western blot analysis.
A. 30 µg HeLa whole cell extract
5 % SDS-PAGE
SAP130 antibody [C3], C-term (107-10624) dilution: 1:10000
A. 30 µg HeLa whole cell extract
5 % SDS-PAGE
SAP130 antibody [C3], C-term (107-10624) dilution: 1:10000
Immunohistochemical analysis of paraffin-embedded Saos-2 xenograft, using SF3B3 (107-10624) antibody at 1:100 dilution.
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.