Rabbit Anti-SIRT3 (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | Q9NTG7 |
| Gene Id | 23410 |
| Gene Symbols | SIRT3 |
| Protein Name / Synonyms | SIRT3 (C-term) |
| Clonality | Polyclonal |
| Preparation | This SIRT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-279 amino acids from the C-terminal region of human SIRT3. |
| Concentration (lot specific) | 0.500 mg/ml |
| Recommended Applications | Western Blotting, IHC-P |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
SIRT3 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The SIRT3 is included in class I of the sirtuin family.
Specificity
Other Names
SIRT3; SIR2L3; NAD-dependent protein deacetylase sirtuin-3, mitochondrial; Regulatory protein SIR2 homolog 3; SIR2-like protein 3
NCBI Accession #
NP_001017524.1;NP_036371.1
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
43573
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100
 | Western blot analysis of lysates from 293, HepG2 cell line (from left to right), using SIRT3 Antibody (C-term)(Cat# 102-16695). 102-16695 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane. |
 | SIRT3 Antibody (C-term) (Cat# 102-16695) western blot analysis in 293 cell lysate (35ug/lane). This demonstrates that the SIRT3 antibody detected SIRT3 protein (arrow). |
 | SIRT3 Antibody (C-term) (Cat# 102-16695) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the SIRT3 antibody detected the SIRT3 protein (arrow). |
 | SIRT3 Antibody (C-term) (Cat# 102-16695) western blot analysis in KG-1 cell line lysates (35ug/lane).This demonstrates the SIRT3 antibody detected the SIRT3 protein (arrow). |
 | Western blot analysis of SIRT3 antibody (Cat# 102-16695) in Y79 cell line lysates (35ug/lane). SIRT3 (arrow) was detected using the purified Pab. |
 | Sirt3 Antibody (G265) (Cat# 102-16695) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the Sirt3 antibody detected the Sirt3 protein (arrow). |
 | Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.