Synonyms
ICA12 antibody, MGC117216 antibody, Sox-13 antibody, SOX13 antibody, type 1 diabetes autoantigen ICA12 antibody, islet cell antibody 12 antibody, SRY-related HMG-box gene 13 antibody, transcription factor SOX-13 antibody, islet cell antigen 12 antibody, SRY-box 13 antibody, SRY (sex determining region Y)-box 13 antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
69 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 75 and 520 of SOX13 (Uniprot ID# Q9UN79)
Applications
Applications Tested
ICC/IF,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunoprecipitation of SOX13 protein from NT2D1 whole cell extracts using 5 µg of SOX13 antibody [N1C3] (107-10439).
Western blot analysis was performed using SOX13 antibody [N1C3] (107-10439).
EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SOX13(107-10439) antibody at 1:200 dilution.

Sample (30 ug of whole cell lysate)
A: Molt-4
7.5% SDS PAGE
107-10439 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.