Rabbit Anti-Human SOX9 (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P48436 |
| Gene Id | 6662 |
| Gene Symbols | SOX9 |
| Protein Name / Synonyms | SOX9 (N-term) |
| Clonality | Polyclonal |
| Preparation | This SOX9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-70 amino acids from the N-terminal region of human SOX9. |
| Concentration (lot specific) | 0.280 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, IF |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
SOX9 recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal.
Specificity
Predicted Reactivity
Mouse, Pig
Other Names
SOX9; Transcription factor SOX-9
NCBI Accession #
NP_000337.1
Other Accession #
O18896; Q04887
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
56137
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; IF: 1:10-50
 | Western blot analysis of anti-SOX9 Antibody (N-term) (Cat# 102-12305) in K562 cell line lysates (35ug/lane). SOX9 (arrow) was detected using the purified Pab. |
 | Western blot analysis of SOX9 (arrow) using rabbit polyclonal SOX9 Antibody (N-term) (Cat# 102-12305).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SOX9 gene (Lane 2) (Origene Technologies). |
 | Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with SOX9 antibody (N-term) (Cat# 102-12305), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Immunofluorescence analysis of anti-SOX9 Antibody (N-term) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.