Rabbit Anti-Human SPI1 (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P17947 |
| Gene Id | 6688 |
| Gene Symbols | SPI1 |
| Protein Name / Synonyms | SPI1 (C-term) |
| Clonality | Polyclonal |
| Preparation | This SPI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 217-246 amino acids from the C-terminal region of human SPI1. |
| Concentration (lot specific) | 0.450 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, IF |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Specificity
Predicted Reactivity
Mouse, Rat, Pig
Other Names
SPI1; Transcription factor PU.1; 31 kDa-transforming protein
NCBI Accession #
NP_001074016.1;NP_003111.2
Other Accession #
Q6BDS1; Q6PKU1; P17433; NP_003111.2; NP_001074016.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
31083
Recommended Dilutions
IF: 1:10-50; WB: 1:1000; IHC: 1:10-50
 | Fluorescent image of NIH/3T3 cell stained with SPI1 Antibody (C-term)(Cat# 102-12686/SA110831AS).NIH/3T3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SPI1 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). SPI1 immunoreactivity is localized to Nucleus significantly. |
 | SPI1 Antibody (C-term) (Cat# 102-12686) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the SPI1 antibody detected the SPI1 protein (arrow). |
 | SPI1 Antibody (C-term) (102-12686)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SPI1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.