Rabbit Anti-Human TBX21 (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q9UL17 |
| Gene Id | 30009 |
| Gene Symbols | TBX21 |
| Protein Name / Synonyms | TBX21 (N-term) |
| Clonality | Polyclonal |
| Preparation | This TBX21 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 22-50 amino acids from the N-terminal region of human TBX21. |
| Concentration (lot specific) | 0.500 mg/ml |
| Recommended Applications | IF, Western Blotting |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human ortholog of mouse Tbx21/Tbet gene. Studies in mouse show that Tbx21 protein is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, interferon-gamma (IFNG). Expression of the human ortholog also correlates with IFNG expression in Th1 and natural killer cells, suggesting a role for this gene in initiating Th1 lineage development from naive Th precursor cells.
Specificity
Other Names
TBX21; TBET; TBLYM; T-box transcription factor TBX21; T-cell-specific T-box transcription factor T-bet; Transcription factor TBLYM
NCBI Accession #
NP_037483.1
Other Accession #
NP_037483.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
58328
Recommended Dilutions
IF: 1:10-50; WB: 1:1000
 | Fluorescent image of A549 cell stained with TBX21 Antibody (N-term)(Cat# 102-12483).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TBX21 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°).TBX21 immunoreactivity is localized to Nucleus significantly. |
 | TBX21 Antibody (N-term) (Cat# 102-12483) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the TBX21 antibody detected the TBX21 protein (arrow). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.